Volume 126, 1 August 2014, Pages 110–115

Kara B. Spilsteada, Jessica J. Leareya, Egan H. Doevena, , , Gregory J. Barbantea, Stephan Mohrb, Neil W. Barnetta, Jessica M. Terrya, Robynne M. Hallc, Paul S. Francisa, , 

a Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3216, Australia

b School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester M60 1QD, UK

c School of Engineering, Deakin University, Geelong, Victoria 3216, Australia

 

Abstract

Herein we explore modern fabrication techniques for the development of chemiluminescence detection flow-cells with features not attainable using the traditional coiled tubing approach. This includes the first 3D-printed chemiluminescence flow-cells, and a milled flow-cell designed to split the analyte stream into two separate detection zones within the same polymer chip. The flow-cells are compared to conventional detection systems using flow injection analysis (FIA) and high performance liquid chromatography (HPLC), with the fast chemiluminescence reactions of an acidic potassium permanganate reagent with morphine and a series of adrenergic phenolic amines.

 

Keywords

Chemiluminescence; Flow-cells; 3D-printing; CNC milling; Acidic potassium permanganate; Flow injection analysis

 

Full text is available at http://www.sciencedirect.com/science/article/pii/S0039914014002288