Journal of Bionic Engineering (2022) 19:1645–1657 https://doi.org/10.1007/s42235-022-00233-w

Chang Liu1  · Dan Deng1  · Jing Gao1  · Shue Jin1  · Yi Zuo1  · Yubao Li1  · Jidong Li1 

Nano-Biomaterials, Analytical & Testing Center, Sichuan University, Chengdu 610064, China

Abstract :The ideal Guided Bone Regeneration (GBR) membrane is required to have good biocompatibility, space maintenance ability, appropriate degradation rate, and preferably can guide the regeneration of vascularized bone tissue. In this study, Simvastatin (SIM) and calcium Phosphate (CaP) were encapsulated in a Polycaprolactone (PCL)/Chitosan (CS) core–shell structural fbrous membranes via coaxial electrospinning technology. The results showed that loaded SIM in the core of the core–shell structure fbrous membranes could sustainably release the drug for more than two months and upregulate the angiogenic marker of Bone Mesenchymal Stem Cells (BMSCs). Adding a certain amount of CaP to the shell layer provided more sites for the mineralization and synergistic with SIM to promote osteogenic diferentiation of BMSCs in vitro. The intramuscular implantation experiments in rabbits suggested a normal early infammation and enhanced vascularization induced by the SIM-loaded fbrous membranes. This study proposed an efective strategy to prepare a dual-loaded core–shell fbrous membrane for guided vascularized bone tissue regeneration. 

Keywords: Simvastatin · Calcium phosphate · Coaxial electrospinning · Vascularization · Guided Bone Regeneration

Schematic diagram of the fabricated functional nanofibrous membranes via coaxial electrospinning